Model development of hydroxyproline induced hyperoxaluria in young growing pigs
DOI:
https://doi.org/10.15584/ejcem.2017.1.1Keywords:
pig model, hyperoxaluria, kidney stones, hydroxyprolineAbstract
AbstractAim of the study. In this study, we sought to create a model of reversible hyperoxaluria in pigs by feeding with hydroxyproline (HP).
Materials and methods. The experiment included 12 pigs divided into 2 groups (n = 6). The pigs were fed twice a day. At the beginning of the experiment, in the adaptation period, all pigs were given standard feed. In the next 7 days, an increasing amount of hydroxyproline (1–3% HP), was added to the feed. In next 14 days, 4% HP was administered in each pig meal. After 14 days of 4% HP diet, the pigs were randomly divided into 2 groups. For 6 pigs, 4% HP treatment had been continued for the next 14 days while the second group of pigs for the next 14 days received a standard HP free diet. 24h urine samples, blood and fecal samples were collected on particular days.
Results. The addition of HP to the diet increased urinary oxalate excretion. A characteristic increase was noted after 12 days of treatment with 4% HP. During the removal period, oxalate excretion decreased in the group without HP in diet, while in the group which continued with a 4% HP diet, oxalate excretion significantly increased. Gross examination of kidneys showed that in the group which had 4% HP diet for 4 weeks, kidneys were fibrotic with enlarged cavities, and had small visible urinary stones. In second group, kidneys were relatively normal looking with no visible stones.
Conclusion. Hyperoxaluria is reversible, if HP is removed 14 days after the start of 4% HP diet. Prolonged exposure up to 4 weeks causes pathologic changes in kidneys including crystals, sand and small stone formation.
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References
Moe O. Kidney stones: pathophysiology and medical management. Lancet. 2006; 367 (9507):333-344
Bar K., Starownik R. Małoinwazyjne metody leczenia kamicy układu moczowego. Przegląd Urologiczny. 2006/7/6 (40)
Sutherland J., Parks J., Coe F. Recurrence after single renal stone in a community practice. Miner electrol metabol. 1985; 11(4):267
Eliahou R., Hidas G., Duvedevani M., Sosna J. Determination of Renal Stone Composition with Duodenal-Energy Computed Tomography: An Emerging Application. Seminars in Ultrasound, CT and MRI. 2010; 31(4): 315 - 320
Harambat J., Fargue S., Bacchetta J., Acquaviva C., Cochat P. Primary Hyperoxaluria. Int J Nephrol. Volume 2011
Hope B., Kemper M., Bökenkamp A., Portale A., Cohn R., Langman C. Plasma oxalate calcium supersaturation in children with primary hyperoxaluria and end stage renal failure. Kidney Int. 1999; 56(1):268-74
Kaplon D., Penniston K., Darriet C., Crenshaw T., Nakada S. Hydroxyproline-induced hyperoxaluria using acidified and traditional diets in the porcine model. J Endourol. 2010; 24(3):355-9.
Mandel N., Henderson J., Hung L., Wille D., Wiessner J. A porcine model of calcium oxalate kidney stone disease. J Urol. 2004; 171:1301–1303
Knight J., Jiang J., Assimos D., Holmes R. Hydroxyproline ingestion and urinary oxalate and glycolate excretion. Kidney Int. 2006; 70(11): 1929 – 1934
Bijarnia R., Kaur T., Singla S., Tandon C. Non-surgical management therapies for kidney stones. J Pharm Educ Res. 2010 June. Issue No. 1
Hatch M., FreelR. Alterations in intestinal transport of oxalate in disease states. Scanning Microsc. 1995;9(4):1121-6; discussion 1126. Review.
Abratt V., Reid S. Oxalate degrading bacteria of the human gut as probiotics in the management of kidney stones disease. Adv Appl Microbiol. 2010;72:63-87. Review.
Cochat P., Hulton S., Acquaviva C., Danpure C., Daudon M., De Marchi M., Fargue S., Groothoff J., Harambat J., Hoppe B., Jamieson N., Kemper M,, Mandrile G., Marangella M., Picca S., Rumsby G., Salido E., Straub M., van Woerden C. Primary Hyperoxaluria Type 1: Indications for screening and guidance for diagnosis and treatment. Nephrol Dial transplant. 2012;27(5):1729-36.
Robinson M., Pond C., Laurie R., Bercz J., Henningsen G., Condie L. Subacute and subchronic toxicity of ethylene glycol administered in drinking water to Sprague–Dawley rats. Drug Chem Toxicity. 1990; 13: 43 – 47.
Khan S., Glenton P., Byer K. Modeling of hyperoxaluric calcium oxalate nephrolithiasis: Experimental induction of hyperoxaluria by hydroxy – L – proline. Kidney Int. 2006; 70:914 – 923.
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