Effect of high fat diet on structure of liver and gallbladder of adult male mice – an experimental study
DOI:
https://doi.org/10.15584/ejcem.2021.4.1Keywords:
COX-2, fatty liver, gallbladder epithelium, immunoexpression, Oil Red O stainingAbstract
AbstractIntroduction. High fat diet (HFD) intake induces obesity and adversely affects different body organs including liver and gallbladder.
Aim. It was to clarify the effects of HFD on the liver and gallbladder structure using light microscopic (LM) examination.
Material and methods. 16 healthy adult male mice were equally divided into 2 groups. Control group mice were fed normal diet. HFD group was fed using HFD. At the end of the 8-week experiment, mice were anesthetized. Liver and gallbladder were removed and prepared to histological processing. Sections were stained with hematoxylin and eosin (H&E) and immunostaining for cyclooxygenase-2 (COX-2) cellular localization. Oil Red O (ORO)-stained frozen liver sections were prepared.
Results. H&E-stained sections of HFD group revealed rounded swollen hepatic cells with pale cytoplasm suggesting cellular ballooning. Dilated congested sinusoids and portal vein, cellular degeneration and collection of inflammatory cells were observed between hepatic cells and in portal region. Gallbladder sections showed epithelial stratification and cellular vacuolation. Strong immunoexpression of COX-2 was observed in Kupffer and hepatic cells of the liver and gallbladder mucosal epithelial cells.
Conclusion. HFD is suggested to alter the normal histological features of liver and gallbladder represented by fatty liver and gallbladder epithelial hyperplasia and inflammatory reaction.
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References
Waugh A, Grant A. Ross and Wilson anatomy and physiology in health and illness. Churchill Livingstone; 2001.
Higashiyama H, Uemura M, Igarashi H, Kurohmaru M, Kanai-Azuma M, Kanai Y. Anatomy and development of the extrahepatic biliary system in mouse and rat: a perspective on the evolutionary loss of the gallbladder. J Anat. 2018;232(1):134-145.
Hegazy A. Clinical embryology for medical students and postgraduate doctors. Lap Lambert Academic Publishing; 2014.
Van der Horst K, Brunner TA, Siegrist M. Fast food and take-away food consumption are associated with different lifestyle characteristics. J Hum Nutr Diet. 2011;24(6):596-602.
Tan Y, Lao W, Xiao L, et al. Managing the combination of nonalcoholic Fatty liver disease and metabolic syndrome with chinese herbal extracts in high-fat-diet fed rats. Evid Based Complement Alternat Med. 2013;2013:306738.
Korish AA, Arafah MM. Camel milk ameliorates steatohepatitis, insulin resistance and lipid peroxidation in experimental non-alcoholic fatty liver disease. BMC Complement Altern Med. 2013;13:264.
Mehlem A, Hagberg CE, Muhl L, Eriksson U, Falkevall A. Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease. Nat Protoc. 2013;8(6):1149-1154.
Mohammed NA, Abd El-Aleem SA, El-Hafiz HA, McMahon RF. Distribution of constitutive (COX-1) and inducible (COX-2) cyclooxygenase in postviral human liver cirrhosis: a possible role for COX-2 in the pathogenesis of liver cirrhosis. J Clin Pathol. 2004;57(4):350-354.
Salinas G, Velásquez C, Saavedra L, et al. Prevalence and risk factors for gallstone disease. Surg Laparosc Endosc Percutan Tech. 2004;14(5):250-253.
Stokes CS, Gluud LL, Casper M, Lammert F. Diets for primary prevention of gallbladder stones in adults. Cochrane Database Syst Rev. 2014;3:1465-1858.
Ruhl CE, Everhart JE. Gallstone disease is associated with increased mortality in the United States. Gastroenterol. 2011;140(2):508-516.
Di Ciaula A, Wang DQ, Bonfrate L, Portincasa P. Current views on genetics and epigenetics of cholesterol gallstone disease. Cholesterol. 2013;2013:298421.
Goldblatt MI, Swartz-Basile DA, Al-Azzawi HH, Tran KQ, Nakeeb A, Pitt HA. Nonalcoholic fatty gallbladder disease: the influence of diet in lean and obese mice. J Gastrointest Surg. 2006;10(2):193-201.
Li X, Gao P. Fatty liver increases gallstone disease risk in younger Chinese patients. Medicine (Baltimore). 2019;98(22):e15940.
Yu J, Ip E, dela Peña A, et al. COX‐2 induction in mice with experimental nutritional steatohepatitis: role as pro-inflammatory mediator. Hepatol. 2006;43(4): 826-836.
Van Erpecum KJ, Wang DQ, Moschetta A, et al. Gallbladder histopathology during murine gallstone formation: relation to motility and concentrating function. J Lipid Res. 2006;47(1): 32-41.
Lavoie B, Nausch B, Zane EA, et al. Disruption of gallbladder smooth muscle function is an early feature in the development of cholesterol gallstone disease. Neurogastroenterol Motil. 2012;24(7):e313-e324.
Wang HH, Portincasa P, Mendez-Sanchez N, Uribe M, Wang DQ. Effect of ezetimibe on the prevention and dissolution of cholesterol gallstones. Gastroenterol. 2008;134(7):2101-2110.
Hegazy R, Hegazy A. Hegazy’simplified method of tissue processing (consuming less time and chemicals). Ann Int Med Dent Res. 2015;1(2):57-61.
Suvarna SK, Layton C, Bancfort JD, Stevens A. Theory and practice of histological techniques, 7thed. Churchill Livingstone. China, 2013.
Kiernan JA. Histological and histochemical methods: Theory and practice,5th ed. Scion. Bloxham, UK, 2015.
Hegazy AA, Ahmed MM, Shehata MA, Abdelfattah MM. Changes in rats’ liver structure induced by zinc oxide nanoparticles and the possible protective role of vitamin E. Int J Hum Anat. 2018;1(3):1-16.
Hegazy AA, Abd Al Hameed EA, El-Wafaey DI, Khorshed OA. Potential role of Moringa Oleifera in alleviating paracetamol-induced nephrotoxicity in rat. Eur J Anat. 2020;24(3):179-191.
Ross MH, Pawlina W. Histology: A Text and Atlas_ with Correlated Cell and Molecular Biology. 7th ed. Wolter Kluwer Health; 2016.
Tsutsumi V, Nakamura T, Ueno T, Torimura T, Aguirre-García J. Structure and ultrastructure of the normal and diseased liver. In Liver Pathophysiology. Muriel, P. (editor). Academic Press; 2017.
Meli R, Mattace Raso G, Irace C, et al. High fat diet induces liver steatosis and early dysregulation of iron metabolism in rats. PLoS One. 2013;8(6):e66570.
Liu J, Zhuang ZJ, Bian DX, et al. Toll-like receptor-4 signalling in the progression of non-alcoholic fatty liver disease induced by high-fat and high-fructose diet in mice. Clin Exp Pharmacol Physiol. 2014;41(7):482-488.
Altunkaynak BZ, Ozbek E, Altunkaynak ME. A stereological and histological analysis of spleen on obese female rats, fed with high fat diet. Saudi Med J. 2007;28(3):353-357.
Arvanitidis AP, Corbett D, Colbourne F. A high fat diet does not exacerbate CA1 injury and cognitive deficits following global ischemia in rats. Brain Res. 2009;1252:192-200.
Elahi MM, Cagampang FR, Mukhtar D, Anthony FW, Ohri SK, Hanson MA. Long-term maternal high-fat feeding from weaning through pregnancy and lactation predisposes offspring to hypertension, raised plasma lipids and fatty liver in mice. Br J Nutr. 2009;102(4):514-519.
Cayuela NC, Negreti GP, Rasslan R, Koike MK, Montero EFS. Oxidative stress on ischemia/reperfusion injury in mice with non-alcoholic hepatic steatosis or steatohepatitis. Acta Cir Bras. 2018;33(9):753-761.
Abu-Amara M, Yang SY, Tapuria N, Fuller B, Davidson B, Seifalian A. Liver ischemia/reperfusion injury: processes in inflammatory networks--a review. Liver Transpl. 2010;16(9):1016-1032.
Kolarski V, Todorov A, Petrova D. Tsitokini i cheren drob pri zdrave i bolest [Cytokines and the liver in health and disease]. Vutr Boles. 2000;32(1):19-24.
Zhang H, Song C, Yan R, Cai H, Zhou Y, Ke X. High-fat diet accelerate hepatic fatty acids synthesis in offspring male rats induced by perinatal exposure to nonylphenol. BMC Pharmacol Toxicol. 2021;27;22(1):22.
Romeijn MM, Kolen AM, Holthuijsen DDB, et al. Effectiveness of a Low-Calorie Diet for Liver Volume Reduction Prior to Bariatric Surgery: A Systematic Review. Obes Surg. 2021;31(1):350-356.
Lindberg MR, Lamps LW. In Diagnostic Pathology: Normal Histology. Lindberg M R. and Lamps LW (Editors). 2nd ed. Elsevier, 2018.
Lammert F, Carey MC, Paigen B. Chromosomal organization of candidate genes involved in cholesterol gallstone formation: a murine gallstone map. Gastroenterol. 2001;120(1):221-238.
Zaki M, Al-Refeidi A. Histological changes in the human gallbladder epithelium associated with gallstones. Oman Med J, 2009;24(4):269-273.
Kakimoto T, Kanemoto H, Fukushima K, Ohno K, Tsujimoto H. Effect of a high-fat-high-cholesterol diet on gallbladder bile acid composition and gallbladder motility in dogs. Am J Vet Res. 2017;78(12):1406-1413.
Murphy MC, Dempsey PJ, Gillespie CD, Murphy AN, McNicholas MMJ. Increased incidence of acute calculous cholecystitis observed during COVID-19 social restrictions. Ir J Med Sci. 2021;1–4. doi: 10.1007/s11845-021-02587-2
Patel NA, Lamb JJ, Hogle NJ, Fowler DL. Therapeutic efficacy of laparoscopic cholecystectomy in the treatment of biliary dyskinesia. Am J Surg. 2004;187(2):209-212.
Guarino MP, Cong P, Cicala M, Alloni R, Carotti S, Behar J. Ursodeoxycholic acid improves muscle contractility and inflammation in symptomatic gallbladders with cholesterol gallstones. Gut. 2007;56(6):815-820.
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