Biochemical and planimetric investigations of hydrophilic creams containing ceramides or dexpanthenol on the model of chemical burns
DOI:
https://doi.org/10.15584/ejcem.2023.2.9Keywords:
ceramides, chemical burns, dexpanthenolAbstract
Introduction and aim. Chemical burns of the skin are common type of injuries both in private life and in industries. Local treatment of chemical burns using wound healing creams and ointments is predominant. Hydrophobic wound healing medicinal products dominate the Ukrainian pharmaceutical market but their hydrophobic base disturbs the healing process of skin. The aim of this work was biochemical and planimetric investigation of treatment efficacy of chemical burns with hydrophilic creams containing ceramides and dexpanthenol.
Material and methods. The experiments were performed on 30 rats weighing 190–220 g. In a rat skin burn model, animals were exposed to 9% acetic acid solution. Treatment was initiated after wound appearance and included application of creams containing ceramides and dexpanthenol. The effectiveness of treatment was estimated using planimetric parameters, such as: surface area of necrotic tissue (S, mm2) and cumulative reparative effect. Levels of the biochemical markers such as total protein, creatinine, C-reactive protein (CRP) and content of SH-groups were measured in the rats’ blood serum.
Results. It was established that cream developed with ceramides and cream with dexpanthenol exhibits reparative properties at the level of 29% and 4.5%, respectively. Biochemical investigations demonstrated the treatment efficacy of creams containing ceramides and dexpanthenol. In terms of CRP level and content of SH-groups, the therapeutic action of cream with dexpanthenol was highly significant by a factor of 1.45 and 1.35, respectively in contrast to the cream with ceramides.
Conclusion. Using the chemical burn model and results of planimetric and biochemical research it was found that cream with ceramides and cream with dexpanthenol exhibit wound-healing properties. In-depth study on the wound-healing mechanism of investigated creams with the aim of creating effective hydrophilic creams for use in burn treatment is prospective.
Downloads
References
Krylov KM, Vagner DO. Zinoviev EV, et al. Life-threatening burns caused by household chemicals. Emergency Medical Care. 2018;7(1):57-61. doi: 10.23934/2223-9022-2018-7-1-57-61
Touzopoulos P, Zarogoulidis P, Mitrakas A, et al. Occupational chemical burns: a 2-year experience in the emergency department. J Multidiscip Healthc. 2011;4:349-352. doi: 10.2147/JMDH.S25141
Robinson E., Chhabra A. Hand chemical burns. J Hand Surg Am. 2015;40(3):605-612. doi: 10.1016/j.jhsa.2014.07.056
Yakovleva L, Tkachova O, Butko Ya, Laryanovska Yu. Experimental study of new medicinal products for local treatment of wounds. DEC MOH of Ukraine: Kyiv, Ukraine. 2013:52.
Butko Y, Tkacheva O, Gorbach T. Oxidizing modification of proteins in case of a burn injury in rats against the topical treatment with new wound healing preparations. Curr Issues Pharm Med Sci. 2013;26(4):372-375. doi: 10.12923/j.2084-980X/26.4/a.03
Gorski J, Proksch E, Baron JM, Schmid D, Zhang L. Dexpanthenol in Wound Healing after Medical and Cosmetic Interventions (Postprocedure Wound Healing). Pharmaceuticals (Basel). 2020;13(7):138. doi: 10.3390/ph13070138
Gheita AA, Gheita TA, Kenawy SA. The potential role of B5: A stitch in time and switch in cytokine. Phytother Res. 2020;34(2):306-314. doi: 10.1002/ptr.6537
Jiang Y, Kim P, Uchida Y, et. al. Ceramides stimulate caspase-14 expression in human keratinocytes. Exp Dermatol. 2013;2(22):113-118. doi: 10.1111/exd.12079
Xu S. Immaneni S, Hazen G, Silverberg J, Paller A, Lio P. Cost-effectiveness of Prophylactic Moisturization for Atopic Dermatitis. JAMA Pediatr. 2017;171(2):e163909. doi: 10.1001/jamapediatrics.2016.3909.
Choi J, Dawe R, Ibbotson S, Fleming C, Doney A, Foerster J. Quantitative analysis of topical treatments in atopic dermatitis: unexpectedly low use of emollients and strong correlation of topical corticosteroid use both with depression and concurrent asthma. Br J Dermatol. 2020;182(4):1017-1025. doi.: 10.1111/bjd.18265
European Convention for the protection of vertebrate animals used for experimental and other scientific purposes (1986). ETS No. 123. Strasbourg. http://conventions.coe.int/treaty/en/treaties/html/123.htm. Accessed 20 January 2023.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 European Journal of Clinical and Experimental Medicine
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our open access policy is in accordance with the Budapest Open Access Initiative (BOAI) definition: this means that articles have free availability on the public Internet, permitting any users to read, download, copy, distribute, print, search, or link to the full texts of these articles, crawl them for indexing, pass them as data to software, or use them for any other lawful purpose, without financial, legal, or technical barriers other than those inseparable from having access to the Internet itself.
All articles are published with free open access under the CC-BY Creative Commons attribution license (the current version is CC-BY, version 4.0). If you submit your paper for publication by the Eur J Clin Exp Med, you agree to have the CC-BY license applied to your work. Under this Open Access license, you, as the author, agree that anyone may download and read the paper for free. In addition, the article may be reused and quoted provided that the original published version is cited. This facilitates freedom in re-use and also ensures that Eur J Clin Exp Med content can be mined without barriers for the research needs.
