Protein microarray – modern technique used in proteomics

Authors

  • Karolina Nowak Z Zakładu Biochemii i Biofarmaceutyków Narodowego Instytutu Leków

Keywords:

proteomics, protein microarray, protein

Abstract

Proteomics is a field of science that studies the structure, function, location of proteins, post-translational modifications and interactions with other proteins. Protein microarray tchnology has become a crucial tool for large-scale and high-throughput research in proteomics. It allows fast, easy and parallel detection of thousands proteins in a single experiment. It has been applied to analyse antibody-antigen, protein-protein, protein-nucleic acid, potein-lipid and proteinsmall molecule interactions, as well as enzyme-substrate interactions. In the past few years, protein microarray technology has shown its great potential in basic research, diagnostics and drug discovery. This article reviews current methods in the generation and applications of protein microarrays.

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References

Węgleński P. i wsp.: Genetyka molekularna. Wyd. Nauk. PWN Warszawa 2002; 25

Hieter P., Boguski M.: Functional genomics: It’s all how you read it. Science 1997; 278:601-602.

Penisi E.: Human genome. Reaching their goal early, sequencing labs celebrate. Science 2003; 300: 409

Ezzell C.: Nadszedł świat białek. Świat Nauki 2002, 30-37

Tomaszewska K., Nalewaj J., Markowska J.: Proteomika – nowa metoda oznaczania markerów nowotworowych. Rak jajnika. Okol.Pol. 2005; 8, 2:79-81

Dmitrzak-Węglarz M., Hauser J.: Wykorzystanie badań protomicznych w poszukiwaniu markerów biologicznych dla chorób psychicznych. Psychiatria 2006; 3:118-127

Wilkins M.R., Sanchez J.C., Gooley A.A. Appel R.D., Humphery-Smith I., Hochstrasser D.F. i wsp.: Progress with proteome projects: why all proteins expressed by genome should be identified and how to do. Biotechnol. Genet. Eng. Rev. 1996; 13: 19-50.

Merrick B.A.: The human proteome organization (HUPO) and environmental health. EHP toxigenomics 2003; 111:1-5

Hanash S.: HUPO Initiatives relevant to clinical Proteomics. MCP 2004; 3: 298-301.

Vercauteren F.G., Bergeron J.J., Vandesande F., Arckens L., Quirion R.: Proteomic Approaches in brain research and neuropharmacology. Eur. J. Pharmacol. 2004; 500: 385-398

Panicker R.C., Chattopadhaya S., Yao S.Q.: Advanced analytical tools in proteomics. Analytica Chimica Act 2006; 556:69-79

Wojtkiewicz K., Nowicka E., Tarnowski R., Widłak P.: Wykorzystanie spektrometrii mas do analizy proteomu surowicy chorych na raka piersi. Onkologia Info 2010; VII, 2:40-47

Watson J.T.: Introduction to Mass Spectrometry, Lippincott-Raven, Philadelphia,PA, 1997.

Zhu H., Snyder M.: Protein chip technology. Curr.Opin.Chem.Biol.2003; 7(1): 55-63.

Bertone P., Snyder M.: Advances in functional protein microarray technology. FEBS J. 2005; 272: 5400-5411.

Zhu H., Bilgin M., Snyder M.: Proteomics. Annu. Rev. Biochem. 2003; 72: 783-812.

Lee Y., Mrksich M.: Protein chips: from concept to practice. Trends Biotechnol. 2002; 20: S14-S18.

MacBeath G., Schreiber S.L.: Printing proteins as microarrays for high-throughput function determination. Science 2000; 289: 1760-1763.

Arnebrant T., Wahlgren M.C.: Protein surfactant interactions at solid surfaces. ACS Sym.Ser. 1995; 602: 239-254.

Kusnezow W., Hoheisel J.D.: Solid suuports for microarray immunoassays. J. Mol. Recognit. 2003; 16 (4): 165-176.

Benters R., Niemeyer C.M., Wohrle D.: Dendrimer-activated solid supports for nucleic acid and protein microarrays. Chembiochem 2001; 2 (9): 686-694.

Schaeferling M., Schiller S., Paul H., Kruschina M., Pavlickowa P., Meerkamp M., Giammasi C., Kambhampati D.: Application of self-assembly techniques in the design of biocompatible protein microarray surfaces. Electrophoresis 2002; 23: 3097-3105.

Cretich M., Damin F., Pirri G., Chiari M.: Protein and peptide arrays: Recent trends and new directions. Biomolecular Engineering 2006; 23: 77-88.

Howbrook D.N., Van der Valk A.M., O’Shaughnessy M.C., Sarker D.K., Baker S.C., Lloyd A.W.: Developments in microarray technologies. DDT 2003; 8 (4); 642-651.

Templin M.F., Stoll D., Schrenk M., Traub P.C., Vohringer C.F., Joos T.O.: Protein microarray technology. Trends Biotech 2002; 20 (4): 160-166.

Espina V., Woodhouse E.C., Wulfkuhle J., Asmussen H.D., Petricoin 3rd E.F., Lotta L.A.: Proetin microarray detection strategies: focus on direct detection technologies. J. Immunol. Methods 2004; 290 (1-2): 121-133.

Cretich M., Damin F., Pirri G., Chiari M.: Protein and peptide arrays: Recent trends and new directions. Biomolecular Engineering 2006; 23: 77-88.

Merchant M., Weinberger S.R.: Recent advancements in surface-enhanced laser desorption/ionization-time of flight-mass spectrometry. Electrophoresis 2000; 21: 1164 -1177.

Ramachandran N., Larson D.N., Stark P.R., Hainsworth E., LaBaer J.: Emerging tools for real-time label-free detection of interactions on functional protein microarrays. FEBS J. 2005; 272 (21): 5412-5425.

Hall D.A., Ptacek J., Snyder M.: Protein microarray technology. Mech. Ageing Dev. 2007; 128: 161-167

Haab B.B.: Antibody arrays in cancer research. Mol. Cell Proteomics 2005; 4: 377-383.

Mezzasoma L., Bacarese-hamilton T., Di Christina M., Rosssi R., Bistoni F., Crisanti A.: Antigen microarrays for serodiagnosis of infectious diseases. Clin. Chem. 2002; 48:121 -130.

Yang L., Guo S., Li Y., Zhou S., Tao S.: Protein microarrays for systems biology. Acta Biochim Biophys Sin 2011; 43(3): 161-171.

Sawiniec J., Borkowski K., Paluszkiewicz P.: Protein microarrays in molecular oncology. Annales UMCS, sect. DDD 2008; 21: 85-89.

Zhu H., Bilgin M., Bangham R., Hall D., Casamayor A., Bertone P., Lan N., Jansen R., Bidlingmaier S., Houfek T., Mitchell T., Miller P., Dean R.A., Gerstein M., Snyder M.: Global analysist of protein activities using proteome chips. Science 2001; 293: 2101-2105.

Krzemiński Z.: Postępy w diagnostyce mikrobiologicznej chorób zakaźnych. Przegl Epidemiol 2003; 57: 377-380.

Published

2011-12-30

How to Cite

Nowak, K. (2011). Protein microarray – modern technique used in proteomics. European Journal of Clinical and Experimental Medicine, 9(4), 523–528. Retrieved from https://journals.ur.edu.pl/ejcem/article/view/12889