Effect of rhIL-17 on the expression of TLR2 and apoptotic proteins in human neutrophils

Authors

  • Magdalena Kakareko Z Zakładu Immunologii Uniwersytetu Medycznego w Białymstoku
  • Ewa Jabłońska Z Zakładu Immunologii Uniwersytetu Medycznego w Białymstoku
  • Jakub Jabłoński Z Zakładu Toksykologii Uniwersytetu Medycznego w Białymstoku
  • Wioletta Ratajczak-Wrona Z Zakładu Immunologii Uniwersytetu Medycznego w Białymstoku

Keywords:

PMN, rhIL-17, Bax, Mcl-1, TLR2

Abstract

Introduction. Neutrophils (PMNs) apoptosis occurs involving proteins of the Bcl-2 family, which control the permeability of the outer mitochondrial membrane. Proteins of this family are regulated by various factors, among others cytokine. Early observations of their own show the relationship between increased apoptosis and expression of Toll-like receptor (TLR2) in these cells stimulated by IL-17.

The aim of the planned study was to investigate the effect of rhIL-17 on selected proteins of the Bcl-2 family, such as: the proapoptotic Bax protein and antiapoptotic Mcl-1 protein in relation to the expression of TLR2 receptor. Reference to the analysed results was to investigate the influence of fMLP on estimated parameters.

Material and method. The study was conducted on human PMN isolated from peripheral blood. Apoptosis was assessed by cytometry, and the expression of TLR2, Bax, and Mcl-1 was estimated by Western blot.

The results and conclusions. The results have shown that the enhancement of neutrophils intense apoptosis depends directly from the effect of rhIL-17 and probably indirectly on TLR2 receptor. The observed changes in the expression of Bax both under the influence of fMLP and rhIL-17 stress the important role of this protein in regulating neutrophils apoptosis.

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Published

2013-03-30

How to Cite

Kakareko, M., Jabłońska, E., Jabłoński, J., & Ratajczak-Wrona, W. (2013). Effect of rhIL-17 on the expression of TLR2 and apoptotic proteins in human neutrophils. European Journal of Clinical and Experimental Medicine, 11(1), 95–105. Retrieved from https://journals.ur.edu.pl/ejcem/article/view/12699

Issue

Vol. 11 No. 1 (2013): Medical Journal of the Rzeszow University and the National Medicines Institute, Warsaw

Section

ORIGINAL PAPERS

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Copyright (c) 2013 Medical Journal of the Rzeszow University and the National Medicines Institute, Warsaw

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