Silica nanoparticles from melon seed husk improves atherogenic, hematologic and oxidative stress indices in male Sprague Dawley rats exposed to Ni, Al and Ni/Al mixtures
DOI:
https://doi.org/10.15584/ejcem.2025.2.11Keywords:
hematotoxicity, lipid profile, nickel and aluminum, oxidative stress, silica nanoparticlesAbstract
Introduction and aim. With the increased awareness from circular bioeconomy that focuses on ‘no waste’ generation mantra, various technologies have been developed to valorize these wastes into useful products, including melon seed husk. The aim of the study was to evaluate the effects of silica nanoparticles from melon seed husk (SiNPs MSH) against Ni, Al, and Ni/Al mixture-induced hematotoxicity and lipotoxicity in Sprague Dawley rats.
Material and methods. Fifty-six male Sprague Dawley, 6 to 8 weeks and weighing 220 to 250 g, were randomly allocated to eight groups (n=7). Group 1 received deionized water only (control), groups 2, 3 and 4 (exposed groups) received the Ni/Al mixture, 0.2 mg/kg Ni and 1.0 mg/kg Al, while groups 5 to 8 received the Ni/Al mixture, Ni, and Al plus 100, 200 and 400 mg/kg of SiNPs respectively for 90 days. Blood samples were collected for biochemical investigation.
Results. Ni, Al and Ni/Al groups showed significant (p<0.05) alteration (p <0.05) in the classic lipid profile, hematological and oxidative stress markers compared to the control. Co-administration with SiNPs did not show significant (p>0.05) difference in these parameters compared to the control.
Conclusion. MSH SiNPs reversed Ni, Al, and Ni/Al mixture mediated hemotoxicity and elevated superoxide dismutase, catalase, and glutathione probably via metal chelation.
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Jiménez-Moreno N, Esparza I, Bimbela F, Gandía LM, Ancín-Azpilicueta C. Valorization of selected fruit and vegetable wastes as bioactive compounds: Opportunities and challenges. Crit Rev Environ Sci Technol. 2019;50(20):2061-2108. doi: 10.1080/10643389.2019.1694819
Castrica M, Rebucci R, Giromini C, Tretola M, Cattaneo D, Baldi A. Total phenolic content and antioxidant capacity of agri-food waste and by-products. Ital J Anim Sci. 2018;18(1):336-341. doi: 10.1080/1828051x.2018.1529544
Ajayi VA, Lateef A. Biotechnological valorization of agrowastes for circular bioeconomy: Melon seed shell, groundnut shell and groundnut peel. Cleaner and Circular Bioeconomy. 2023;4:100039. doi: 10.1016/j.clcb.2023.100039
Mehmood U. Contribution of renewable energy towards environmental quality: The role of education to achieve sustainable development goals in G11 countries. Renewable Energy. 2021;178:600-607. doi: 10.1016/j.renene.2021.06.118
Joshi PVK. Fruit and Vegetable Processing Waste Management- An Overview. International Journal of Food and Fermentation technology. 2020;10(2). doi: 10.30954/22779396.02.2020
Andrade-Filho JD, Scholte RGC, Amaral ALG, Shimabukuro PHF, Carvalho OS, Caldeira RL. Occurrence and Probability Maps of Lutzomyia longipalpis and Lutzomyia cruzi (Diptera: Psychodidae: Phlebotominae) in Brazil. Journal of Medical Entomology. 2017;54(5):1430-1434. doi: 10.1093/jme/tjx094
Rahman MM, Howladar MF, Hossain MA, Shahidul Huqe Muzemder ATM, Al Numanbakth MA. Impact assessment of anthropogenic activities on water environment of Tillai River and its surroundings, Barapukuria Thermal Power Plant, Dinajpur, Bangladesh. Groundwater for Sustainable Development. 2020;10:100310. doi: 10.1016/j. gsd.2019.100310
Anyanwu B, Ezejiofor A, Igweze Z, Orisakwe O. Heavy Metal Mixture Exposure and Effects in Developing Nations: An Update. Toxics. 2018;6(4):65. doi: 10.3390/toxics6040065
Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN. Toxicity, mechanism and health effects of some heavy metals. Interdisciplinary Toxicology. 2014;7(2):60-72. doi: 10.2478/intox-2014-0009
Nguyen NT, Wereley ST, Wereley ST, Mousavi A. Fundamentals and Applications of Microfluidics, Third Edition. USA; 2019.
Mehrabadi S. Effects of Chronic Administration of Nickel on Memory Function, Hippocampal Neuronal Morphology and Oxidative Stress Factors in Male Adult Rats. Archives of Advances in Biosciences. 2022;13:1-8.
Zghari O, Rezqaoui A, Ouakki S, et al. Effect of Chronic Aluminum Administration on Affective and Cognitive Behavior in Male and Female Rats. Journal of Behavioral and Brain Science. 2018;08(4):179-196. doi: 10.4236/jbbs.2018.84012
Parmar HS, Kar A. Protective role of Mangifera indica, Cucumis Melo and Citrullus vulgaris peel extracts in chemically induced hypothyroidism. Chem Biol Interact. 2018;177(3):254-258.
Al-Otaibi SN, Alshammari GM, AlMohanna FH, Al-Khalifa AS, Yahya MA. Antihyperlipidemic and hepatic antioxidant effects of Leek leaf methanol extract in high fat diet-fed rats. All Life. 2020;13(1):373-385. doi: 10.1080/26895293.2020.1792355
Abo Ghanima MM, Elsadek MF, Taha AE, et al. Effect of Housing System and Rosemary and Cinnamon Essential Oils on Layers Performance, Egg Quality, Haematological Traits, Blood Chemistry, Immunity, and Antioxidant. Animals. 2020;10(2):245. doi: 10.3390/ani10020245
Moghadam SK, Mahnaz Tabibiazar, Behzad Masoumi, Ahmadi P, Azar ST. Characterization of Whey Protein Isolate-Ascorbic Acid stabilized Oil in Water Pickering Emulsions. Journal of Agriculture and Food Research. 2025:101650-101650. doi: 10.1016/j.jafr.2025.101650
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry. 1979;95(2):351-358. doi: 10.1016/0003-2697(79)90738-3
Salter M, Knowles RG. Assay of NOS activity by the measurement of conversion of oxyhemoglobin to methemoglobin by NO. Methods Mol Biol. 1998;100:61-65. doi:10.1385/1-59259-749-1:61
Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972;247(10):3170-3175.
Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR. Bromobenzene-Induced Liver Necrosis. Protective Role of Glutathione and Evidence for 3,4-Bromobenzene Oxide as the Hepatotoxic Metabolite. Pharmacology. 1974;11(3):151-169. doi: 10.1159/000136485
Claiborne A, Mallett TC, Yeh JI, Luba J, Parsonage D. Structural, redox, and mechanistic parameters for cysteine-sulfenic acid function in catalysis and regulation. Adv Protein Chem. 2001;58:215-276. doi: 10.1016/s0065-3233(01)58006-7
Turner MC, Andersen ZJ, Baccarelli A, et al. Outdoor air pollution and cancer: An overview of the current evidence and public health recommendations. CA: A Cancer Journal for Clinicians. 2020;70(6). doi: 10.3322/caac.21632
Klein CB and Costa MAX. Nickel. In Handbook on the Toxicology of Metals. 615-637. Academic Press. Ghoma WEO, Sevik H, Isinkaralar K. Using indoor plants as biomonitors for detection of toxic metals by tobacco smoke. Air Quality, Atmosphere and Health. 2022:15(3):415-424.
Goswami R, Neog N. Heavy metal pollution in the environment: impact on air quality and human health implications. In Heavy metal toxicity; Environmental concerns, remediation, and opportunities. 2023;75-103. Singapore: Springer Nature Singapore.
Tietz T, Lenzner A, Kolbaum AE, et al. Aggregated aluminium exposure: risk assessment for the general population. Archives of Toxicology. 2019;93(12):3503-3521. doi: 10.1007/s00204-019-02599-z
Sanajou S, Şahin G, Baydar T. Aluminium in cosmetics and personal care products. J Appl Toxicol. 2021;41(11):1704-1718. doi:10.1002/jat.4228
Almukainzi M, Alotaibi L, Abdulwahab A, Albukhary N, El Mahdy AM. Quality and safety investigation of commonly used topical cosmetic preparations. Sci. Rep. 2022;12(1). doi: 10.1038/s41598-022-21771-7
Mitra S, Chakraborty AJ, Tareq AM, et al. Impact of Heavy Metals on the Environment and Human health: Novel Therapeutic Insights to Counter the Toxicity. Journal of King Saud University - Science. 2022;34(3):101865. doi: 10.1016/j.jksus.2022.101865
Sule K, Umbsaar J, Prenner EJ. Mechanisms of Co, Ni, and Mn toxicity: From exposure and homeostasis to their interactions with and impact on lipids and biomembranes. Biochim Biophys Acta Biomembr. 2020;1862(8):183250. doi: 10.1016/j.bbamem.2020.183250
Górska A, Markiewicz-Gospodarek A, Chilimoniuk Z, Kuszta P, Czarnek K. The Effects of the Action of Chromium, Aluminum, Nickel and Iron on Human Fibroblast and Stem Cell Cultures. Teka Komisji Prawniczej PAN Oddział w Lublinie. 2022;15(2):131-151. doi: 10.32084/tkp.5143
Zhou Y, Addai FP, Zhang X, Liu Y, Wang Y, Lin F, Shi H. Heavy metal-induced lipogenic gene aberration, lipid dysregulation and obesogenic effect: a review. Environmental Chemistry Letters. 2022;20(3):1611-1643.
Gaur A, Nayak P, Ghosh S, Sengupta T, Sakthivadivel V. Aluminum as a Possible Cause Toward Dyslipidemia. Indian Journal of Occupational and Environmental Medicine. 2023;27(2):112-119. doi: 10.4103/ijoem.ijoem_349_21
Zhou S, Li H, Wang H, et al. Nickel Nanoparticles Induced Hepatotoxicity in Mice via Lipid-Metabolism-Dysfunction-Regulated Inflammatory Injury. Molecules. 2023;28(15):5757-5757. doi: 10.3390/molecules28155757
Renu K, Chakraborty R, Myakala H, et al. Molecular mechanism of heavy metals (Lead, Chromium, Arsenic, Mercury, Nickel and Cadmium) - induced hepatotoxicity – A review. Chemosphere. 2021;271:129735. doi: 10.1016/j.chemosphere.2021.129735
You M, Arteel GE. Effect of ethanol on lipid metabolism. Journal of Hepatology. 2019;70(2):237-248. doi: 10.1016/j.jhep.2018.10.037
Agbu P, Carthew RW. MicroRNA-mediated regulation of glucose and lipid metabolism. Nat Rev Mol Cell Biol. 2021;22(6):425-438. doi:10.1038/s41580-021-00354-w
Bhardwaj H, Singh C, Nayyar S. Assessment of Adverse Effects of Lead, Nickel and Cadmium on Biochemical Parameters, Antioxidants Status and Metallothionein Expression in Buffaloes Slaughtered at Local Abattoir. Indian Journal of Animal Research. 2021. doi: 10.18805/ijar.b-4242
Rakib MA, Ali M, Akter MS, Bhuiyan MA. Assessment of heavy metal (Pb, Zn, Cr and Cu) content in roadside dust of Dhaka Metropolitan City, Bangladesh. Int Res J Environ Sci. 2014;3(1):1-5.
Koleva P, Bridgman S, Kozyrskyj A. The Infant Gut Microbiome: Evidence for Obesity Risk and Dietary Intervention. Nutrients. 2015;7(4):2237-2260. doi: 10.3390/nu7042237
Khazaal AS, Zakari MG. Prevalence of Obesity-Related Health Disorders Detected During Preoperative Evaluation for Bariatric Surgery. Prevalence. 2021;44:6.
Fu Z, Xi S. The effects of heavy metals on human metabolism. Toxicology Mechanisms and Methods. 2020;30(3):167176. doi: 10.1080/15376516.2019.1701594
Silveira Rossi JL, Barbalho SM, Reverete de Araujo R, Bechara MD, Sloan KP, Sloan LA. Metabolic syndrome and cardiovascular diseases: Going beyond traditional risk factors. Diabetes/Metabolism Research and Reviews. 2021;38(3). doi: 10.1002/dmrr.3502
Fawwad A, Mahmood Y, Askari S, et al. NDSP 12: Atherogenic index of plasma as a useful marker of cardiovascular disease risk among Pakistani individuals; a study from the second National Diabetes Survey of Pakistan (NDSP) 2016–2017. Clinical Epidemiology and Global Health. 2023;19:101202. doi: 10.1016/j.cegh.2022.101202
Yang F, Smith MJ. Metal profiling in coronary ischemia-reperfusion injury: Implications for KEAP1/NRF2 regulated redox signaling. Free Radic Biol Med. 2024;210:158-171. doi: 10.1016/j.freeradbiomed.2023.11.013
Shun CH, Yuan TH, Hung SH, Yeh YP, Chen YH, Chan CC. Assessment of the hyperlipidemia risk for residents exposed to potential emitted metals in the vicinity of a petrochemical complex. Environ Sci Pollut Res Int. 2021;28(22):27966-27975. doi: 10.1007/s11356-021-12642-1
Nasab H, Rajabi S, Eghbalian M, Malakootian M, Hashemi M, Mahmoudi-Moghaddam H. Association of As, Pb, Cr, and Zn urinary heavy metals levels with predictive indicators of cardiovascular disease and obesity in children and adolescents. Chemosphere. 2022;294:133664. doi: 10.1016/j.chemosphere.2022.133664
Wang R, Huang Y, Yu L, et al. The role of mitochondrial dynamics imbalance in hexavalent chromium-induced apoptosis and autophagy in rat testis. Chemico-Biological Interactions. 2023;374:110424-110424. doi: 10.1016/j.cbi.2023.110424
Li H, Kilgallen AB, Münzel T, et al. Influence of mental stress and environmental toxins on circadian clocks: Implications for redox regulation of the heart and cardioprotection. Br J Pharmacol. 2020;177(23):5393-5412. doi:10.1111/bph.14949
Molani Gol R, Rafraf M, Asghari Jafarabadi M. Assessment of atherogenic indices and lipid ratios in the apparently healthy women aged 30–55 years. Arterial Hypertension. 2022;25(4):172-177. doi: 10.5603/ah.a2021.0020
Sniderman AD, Thanassoulis G, Glavinovic T, et al. Apolipoprotein B Particles and Cardiovascular Disease: A Narrative Review. JAMA Cardiol. 2019;4(12):1287-1295. doi: 10.1001/jamacardio.2019.3780
Alsaweed M. Oxidative Modification of Lipoproteins: A Potential Role of Oxidized Small dense LDL in Enhanced Atherogenicity. Journal of Pharmaceutical Research International. 2021:118-140. doi: 10.9734/jpri/2021/v33i47a32997
Jin X, Yang S, Lu J, Wu M. Small, Dense Low-Density Lipoprotein-Cholesterol and Atherosclerosis: Relationship and Therapeutic Strategies. Front Cardiovasc Med. 2022;8. doi: 10.3389/fcvm.2021.804214
Zhang XH, Ma C, Zhang L, et al. GR24-mediated enhancement of salt tolerance and roles of H2O2 and Ca2+ in regulating this enhancement in cucumber. Journal of Plant Physiology. 2022;270:153640-153640. doi: 10.1016/j.jplph.2022.153640
Briffa J, Sinagra E, Blundell R. Heavy Metal Pollution in the Environment and Their Toxicological Effects on Humans. Heliyon. 2020;6(9):e04691. doi: 10.1016/j.heliyon.2020.e04691
Hembrom S, Singh B, Gupta SK, Nema AK. A Comprehensive Evaluation of Heavy Metal Contamination in Foodstuff and Associated Human Health Risk: A Global Perspective. Contemporary Environmental Issues and Challenges in Era of Climate Change. Published online November 17, 2019:33-63. doi: 10.1007/978-981-32-9595-7_2
Mulware SJ. Toxicity of Heavy Metals, A. Subject in Review. International Journal Recent Research in Physics and Chemical Sciences. 2020;6(2):30-43
Bansal HN. Heavy metal toxicity: A comprehensive review of forms, exposure routes, toxicokinetics, and effects on infants. International Journal of Medical Toxicology and Legal Medicine. 2023;26(1 and 2):13-24. doi: 10.5958/09744614.2023.00004.9
Gwozdzinski K, Pieniazek A, Gwozdzinski L. Reactive Oxygen Species and Their Involvement in Red Blood Cell Damage in Chronic Kidney Disease. Oxid Med Cell Longev. 2021;2021:6639199. doi: 10.1155/2021/6639199
Alfhili M, Alamri H, Jawaher Alsughayyir, Basudan A. Induction of hemolysis and eryptosis by occupational pollutant nickel chloride is mediated through calcium influx and p38 MAP kinase signaling. International Journal of Occupational Medicine and Environmental Health. 2021;35(1):1-11. doi: 10.13075/ijomeh.1896.01814
Pathak A, Singh SP. Mitigating Lead and Nickel Toxicity: Ameliorative Effects of Cichorium intybus Extract in Wistar Rats. Journal of Veterinary Pharmacology and Toxicology, 2022;21(2):14-20.
Neves J, Haider T, Gassmann M, Muckenthaler MU. Iron Homeostasis in the Lungs—A Balance between Health and Disease. Pharmaceuticals. 2019;12(1):5. doi: 10.3390/ph12010005
Burgos-Aceves MA, Lionetti L, Faggio C. Multidisciplinary haematology as prognostic device in environmental and xenobiotic stress-induced response in fish. Sci Total Environ. 2019;670:1170-1183. doi: 10.1016/j.scitotenv.2019.03.275
Ravingerová T, Kindernay L, Barteková M, et al. The Molecular Mechanisms of Iron Metabolism and Its Role in Cardiac Dysfunction and Cardioprotection. Int J Mol Sci. 2020;21(21):7889. doi: 10.3390/ijms21217889
Hante NK, Medina C, Santos-Martinez MJ. Effect on Platelet Function of Metal-Based Nanoparticles Developed for Medical Applications. Front Cardiovasc Med. 2019;6. doi: 10.3389/fcvm.2019.00139
Gonzalez-Villalva A, Bizarro-Nevares P, Rojas-Lemus M, et al. A brief review of the biology of megakaryocytes and platelets and their role in thrombosis associated with particulate air pollution. Toxicology and Industrial Health. 2021;37(3):164-172. doi: 10.1177/0748233720986352
Han M, Lin W, Huang S, Lin Z, Li K. Association between Plasma Metal Elements and Platelet Dysfunction in Trauma-Induced Coagulopathy Rat Model. SSRN Electronic Journal. 2022. doi: 10.2139/ssrn.4028390
Karimi Z, Alizadeh AM, Dolatabadi JEN, Dehghan P. Nigella sativaand its derivatives as food toxicity protectant agents. Advanced pharmaceutical bulletin. 2019;9(1).
Kerdsomboon K, Chumsawat W, Auesukaree C. Effects of Moringa oleifera leaf extracts and its bioactive compound gallic acid on reducing toxicities of heavy metals and metalloid in Saccharomyces cerevisiae. Chemosphere. 2021;270:128659. doi: 10.1016/j.chemosphere.2020.128659
Mihailovic V, Katanic Stankovic JS, Selakovic D, Rosic G. An Overview of the Beneficial Role of Antioxidants in the Treatment of Nanoparticle-Induced Toxicities. Oxid Med Cell Longev. 2021;2021:7244677. doi: 10.1155/2021/7244677
Dhalaria R, Verma R, Kumar D, et al. Bioactive Compounds of Edible Fruits with Their Anti-Aging Properties: A Comprehensive Review to Prolong Human Life. Antioxidants. 2020;9(11):1123. doi: 10.3390/antiox9111123
Barbouti A, Lagopati N, Veroutis D, et al. Implication of dietary iron-chelating bioactive compounds in molecular mechanisms of oxidative stress-induced cell ageing. Antioxidants. 2021;10(3):491. doi: 10.3390/antiox10030491
Pisoschi AM, Pop A, Iordache F, Stanca L, Predoi G, Serban AI. Oxidative stress mitigation by antioxidants – An overview on their chemistry and influences on health status. Eur J Med Chem. 2021;209:112891. doi: 10.1016/j.ejmech.2020.112891
Anyachor CP, Orish CN, Ezejiofor AN, et al. Ni and Al mixture amplifies cerebellar oxido-inflammatory responses, down regulates AChE and BDNF/NGF levels in motor impairment in male albino rats. Journal of Trace Elements in Medicine and Biology. 2023;80:127318-127318. doi: 10.1016/j.jtemb.2023.127318
Marzban A, Seyedalipour B, Mianabady M, Taravati A, Hoseini SM. Biochemical, toxicological, and histopathological outcome in rat brain following treatment with NiO and NiO nanoparticles. Biological trace element research, 2020;196:528-536.
Iqbal S, Jabeen F, Peng C, Ijaz M, Chaudhry A. Cinna- momum cassia ameliorates Ni-NPs-induced liver and kidney damage in male Sprague Dawley rats. Human & Experimental Toxicology. 2020;39(11):1565-1581. doi: 10.1177/0960327120930125
Balali-Mood M, Naseri K, Tahergorabi Z, Khazdair MR, Sadeghi M. Toxic Mechanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium, and Arsenic. Front Pharm. 2021;12:643972. doi: 10.3389/fphar.2021.643972
Naresh Dumala, Bhanuramya Mangalampalli, Srinivas S, Grover P. Repeated oral dose toxicity study of nickel oxide nanoparticles in Wistar rats: a histological and biochemical perspective. J Appl Toxicol. 2019;39(7):1012-1029. doi: 10.1002/jat.3790
Apiamu A, Avwioroko OJ, Evuen UF, et al. Exposure to Nickel–Cadmium Contamination of Drinking Water Culminates in Liver Cirrhosis, Renal Azotemia, and Metabolic Stress in Rats. Biol Trace Elem Res. 2024;202(4):1628-1643. doi: 10.1007/s12011-023-03777-y
Liaquat L, Sadir S, Batool Z, et al. Acute aluminum chloride toxicity revisited: Study on DNA damage and histopathological, biochemical and neurochemical alterations in rat brain. Life Sciences. 2019;217:202-211. doi: 10.1016/j.lfs.2018.12.009
El‐Shetry ES, Mohamed AA, Khater SI, et al. Synergistically enhanced apoptotic and oxidative DNA damaging pathways in the rat brain with lead and/or aluminum metals toxicity: Expression pattern of genes OGG1 and P53. J Trace Elem Med Biol. 2021;68:126860-126860. doi: 10.1016/j.jtemb.2021.126860
Ogunlade B, Adelakun SA, Agie JA. Nutritional supplementation of gallic acid ameliorates Alzheimer-type hippocampal neurodegeneration and cognitive impairment induced by aluminum chloride exposure in adult
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