Wound healing potential of Apamarga Ksharodaka (herbal alkaline water made from Achyranthus aspera Linn.) in excision rodent wound model
DOI:
https://doi.org/10.15584/ejcem.2025.1.23Keywords:
antimicrobial activity, Apamarga ksharodaka, excision wound model, phytochemical richness, traditional medicinal plants, wound, wound healingAbstract
Introduction and aim. Wound healing is a biological process that aims to restore tissue integrity and function. Despite medical advances, wound management remains challenging. Traditional medicinal preparations, like Apamarga ksharodaka (AK), offer promising therapeutic potential due to their phytochemical richness. This study evaluated wound healing and antimicrobial activity of AK. This study aimed to validate the traditional claim of AK’s wound healing potential using an excision wound model.
Material and methods. An excision wound model was created using 24 male Wistar rats. A positive control group applied 5% w/w povidone-iodine (PI) ointment. Wound contraction (WC), epithelialization period (ET), wound closure day, and histopathology were assessed. Antibacterial activity was evaluated against Escherichia coli, Streptococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii.
Results. AK showed slightly better wound healing than PI ointment, with significant results in WC rate, wound closure, and ET. Histopathology revealed normal skin and organ architecture. The minimum MIC was 6.25 mg/ml against Pseudomonas aeruginosa with a maximum inhibition zone of 15 mm.
Conclusion. AK is safe and effective for wound healing.
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Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res. 2009;37(5):1528-1542. doi: 10.1177/147323000903700531
Woo KY, Krasner DL, Kennedy B, Wardle D, Moir O. Palliative wound care management strategies for palliative patients and their circles of care. Adv Skin Wound Care. 2015;28(3):130-140. doi: 10.1097/01.ASW.0000461116.13218.43.
Jadav HR, Galib R, Prajapati PK. Pharmaceutical standardization of Apamarga kshara. J Ayurveda Integr Med. 2015;6(4):290. doi: 10.4103/0975-9476.172387.
Akbar S, Akbar S. Achyranthes aspera L.(Amaranthaceae). Handb 200 Med Plants A Compr Rev Their Tradit Med Uses Sci Justifications. 2020:69-80.
Chouhan P, Garg AK. Agrochemical exposure and its adverse effects on pregnancy with the importance of preconceptional detoxification and management through ayurveda. J Ayurveda 2021;15:303-310. doi: 10.4103/joa.joa_189_20
Mahapatra A, Srinivasan A, Sujithra R, Bhat RP. Management of internal hemorrhoids by Kshara karma: An educational case report. J Ayurveda Integr Med. 2012;3(3):115. doi: 10.4103/0975-9476.100169.
Chouhan P, Garg AK. Ayurvedic management of infertility W.S.R. to tubal blockage by Kshar Taila Uttar Basti: A case study. J Ayurveda. 2024;18:240-244. doi: 10.4103/joa.joa_363_21
Gundeti MS, Reddy RG, Muralidhar JV. Subcutaneous intralesional Ksharodaka injection: A novel treatment for the management of Warts: A case series. J Ayurveda Integr Med. 2014;5(4):236. doi: 10.4103/0975-9476.146558
Balbhadra S, Ganti BY, Narayana SKK. Monographic Analysis of Apamarga Kshara Preparation Methods Mentioned In Five Different Ayurvedic Texts. J Ayurveda Med Sci. 2018;3(1). doi: 10.5530/jams.2018.3.9
Dutta G, Bain J, Ray AK, Dey S, Das N, Das B. Comparing Ksharasutra (Ayurvedic Seton) and open fistulotomy in the management of fistula-in-ano. J Nat Sci Biol Med. 2015;6(2):406. doi: 10.4103/0976-9668.160022
Jahandideh M, Hajimehdipoor H, Mortazavi SA, Dehpour A, Hassanzadeh G. Evaluation of the wound healing activity of a traditional compound herbal product using rat excision wound model. Iran J Pharm Res IJPR. 2017;16:153.
Lindholm C, Searle R. Wound management for the 21st century: combining effectiveness and efficiency. Int Wound J. 2016;13:5-15. doi: 10.1111/iwj.12623
Chouhan P, Garg AK. Exploring The Therapeutic Potential of Herbal Intervention in Polycystic Ovary Syndrome: A Systemic Review and Meta-Analysis. Migrat Lett 2023;20(12):1308-1333. doi: 10.59670/ml.v20is12.8536
Mohite M, Tiwari MD, Kalmegh MS, Mudgal R, Bawa RR. A Comparative Evaluation of Antimicrobial Effect of Snuhi-Apamarg-Ksharsutra and Udumbara-Ksheer Sutra in the Management of Bhagandara (Fistula-In-Ano). Int J Ayurvedic Med. 2023;14(4):1015-1020. doi: 10.47552/ijam.v14i4.3965
Chouhan P, Garg AK. Ovulation induction in polycystic ovarian syndrome: A review to contemporary approaches. Journal of Medical Society. 2023;37(2):45-50. doi: 10.4103/jms.jms_21_22
Mengie T, Mequanente S, Nigussie D, Legesse B, Makonnen E. Investigation of wound healing and anti-inflammatory activities of solvent fractions of 80% methanol leaf extract of Achyranthes aspera L. (Amaranthaceae) in rats. J Inflamm Res. 2021:1775-1787. doi: 10.2147/JIR.S298244
Wallace LA, Gwynne L, Jenkins T. Challenges and opportunities of pH in chronic wounds. Ther Deliv. 2019;10(11):719-735. doi: 10.4155/tde-2019-0066
Pastar I, Stojadinovic O, Yin NC, et al. Epithelialization in wound healing: a comprehensive review. Adv wound care. 2014;3(7):445-464. doi: 10.1089/wound.2013.0473
Childs DR, Murthy AS. Overview of wound healing and management. Surg Clin. 2017;97(1):189-207. doi: 10.1016/j.suc.2016.08.013
Soforowa EA. Medicinal plants and traditional medicine in Africa. John Willey sons, New York. 1982. doi: 10.4236/fns.2015.615144
Yadav R, Rai R, Yadav A, Pahuja M, Solanki S, Yadav H. Evaluation of antibacterial activity of Achyranthes aspera extract against Streptococcus mutans: An: in vitro: study. J Adv Pharm Technol Res. 2016;7(4):149-152. doi: 10.4103/2231-4040.191426
Kaur M, Thakur Y, Rana RC. Antimicrobial properties of Achyranthes aspera. Anc Sci Life. 2005;24(4):168-173.
Owk AK, Mortha KR, Lagudu MN. Evaluation of antimicrobial activity of chemical constituents of Achyranthes aspera L. roots against human pathogens. Indian J Nat Prod Resour (IJNPR)[Formerly Nat Prod Radiance (NPR)]. 2015;5(3):278-281. doi: 10.56042/ijnpr.v5i3.4699
Dubey S, Singh A, Ojha KK, et al. Recent trends in wound care herbal formulations: meta-analysis and systematic review. Int J Pharm Sci & Res. 2023;14(4):1561-1571. doi: 10.13040/IJPSR.0975-8232
Edwin S, Jarald EE, Deb L, et al. Wound healing and antioxidant activity of Achyranthes aspera. Pharm Biol. 2008;46(12):824-828. doi: 10.1080/13880200802366645
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