Morphology of extensor digitorum longus of Wistar rats after remobilization by vibratory platform
DOI:
https://doi.org/10.15584/ejcem.2019.4.2Keywords:
immobilization, skeletal muscle, vibrationAbstract
Introduction. In exercise, vibrations are performed in order to produce rapid and short changes in muscle length. These changes are detected by sensory receptors, in response try to dampen the vibratory waves through a modulation of muscle stiffness. However, its effects on the morphology of muscle tissue are still not fully established, especially after long periods of immobilization.
Aim. To compare the effects of the vibratory platform on the remobilization of the extensor digitorum longus (EDL) muscle of Wistar rats with free remobilization.
Material and methods. 20 rats were divided into: CG (Control), IG (immobilized), IFG (immobilization and free remobilization), IPG (immobilization and remobilization with vibratory platform). The immobilization was performed on the pelvic limb for 15 days. The remobilization with vibratory platform was done for 10 minutes daily, for 2 weeks. The EDL was processed for histological analysis of cross-sections.
Results. The area, larger diameter, smaller diameter and fiber density of the EDL muscle of GI presented significant alteration when opposed to CG, IFG and IPG. The density of nuclei of the EDL muscle of IG presented a significant increase when opposed to the others, and IPG also presented a significant increase when compared to CG.
Conclusion. The morphology and morphometry of the EDL muscle tissue were affected, and both free and vibration platform remobilization re-established the morphological aspects of the muscle fiber, without significant differences between the methods.
Downloads
References
Kunz RI, Coradini JG, Silva LI, et al. Morfologia dos músculos sóleo e tibial anterior de ratos Wistar imobilizados e remobilizados em meio aquático. Conscientiae Saúde. 2014;13(4):595-602.
Lieber RL, Roberts TJ, Blemker SS, Lee SSM, Herzog W. Skeletal muscle mechanics, energetics and plasticity. J Neuroeng Rehabil. 2017;14(1):108.
Hoppeler H. Molecular networks in skeletal muscle plasticity. J Exp Biol. 2016;219(2):205-213.
Kjøbsted R, Hingst JR, Fentz J, et al. AMPK in skeletal muscle function and metabolism. FASEB J. 2019;32(4):1741-1777.
Hood DA. Coordination of metabolic plasticity in skeletal muscle. J Exp Biol. 2006;209(12):2265-2275.
Zhang P, Chen X, Fan M. Signaling mechanisms involved in disuse muscle atrophy. Med Hypotheses. 2007;69(2):310-321.
Devries MC, Phillips SM. Supplemental protein in support of muscle mass and health: advantage whey. J Food Sci. 2015;80(S1):A8-A15.
Morley JE. Hormones and sarcopenia. Curr Pharm Des. 2017;23(30):4484-4492.
Cornachione AS, Cação-Benedini LO, Benedini-Elias PCO, Martinez EZ, Mattiello-Sverzut AC. Effects of 40 min of maintained stretch on the soleus and plantaris muscles of rats applied for different periods of time after hindlimb immobilization. Acta Histochem. 2013;115:505-511.
Baroni BM, Galvão AQ, Ritzel CH, Diefenthaeler F, Vaz MA. Adaptações neuromusculares de flexores dorsais e plantares a duas semanas de imobilização após entorse de tornozelo. Rev Bras Med do Esporte. 2010;16(5):358-362.
Atherton PJ, Greenhaff PL, Phillips SM, Bodine SC, Adams CM, Lang CH. Control of skeletal muscle atrophy in response to disuse: clinical/preclinical contentions and fallacies of evidence. Am J Physiol Endocrinol Metab. 2016;311(3):E594–E604.
Andrushko JW, Lanovaz JL, Björkman KM, Kontulainen SA, Farthing JP. Unilateral strength training leads to muscle-specific sparing effects during opposite homologous limb immobilization. J Appl Physiol. 2018;124(4):866-876.
Gao Y, Arfat Y, Wang H, Goswami N. Muscle atrophy induced by mechanical unloading: mechanisms and potential countermeasures. Front Physiol. 2018;9:235.
Rocha WA, Gobbi GA, Araujo V de F, et al. Alterações morfofuncionais musculares em resposta ao alongamento passivo em modelo animal de imobilização prolongada de membro posterior. Rev Bras Med do Esporte. 2010;16(6):450-454.
Flieger J, Karachalios T, Khaldi L, Raptou P, Lyritis G. Mechanical stimulation in the form of vibration prevents postmenopausal bone loss in ovariectomized rats. Calcif Tissue Int. 1998;63(6):510-514.
Wollersheim T, Haas K, Wolf S, et al. Whole-body vibration to prevent intensive care unit-acquired weakness: safety, feasibility, and metabolic response. Crit Care. 2017;21(1):9.
Fratini A, Bonci T, Bull AMJ. Whole body vibration treatments in postmenopausal women can improve bone mineral density: Results of a stimulus focussed meta-analysis. PLoS One. 2016;11(12):e0166774.
Rogan S, Taeymans J, Radlinger L, et al. Effects of whole-body vibration on postural control in elderly: An update of a systematic review and meta-analysis. Arch Gerontol Geriatr. 2017;73:95-112.
Aoyama A, Yamaoka-Tojo M, Obara S, et al. Acute effects of whole-body vibration training on endothelial function and cardiovascular response in elderly patients with cardiovascular disease. Int Heart J. 2019;60(4):854-861.
Chang S-F, Lin P-C, Yang R-S, Yang R-J. The preliminary effect of whole-body vibration intervention on improving the skeletal muscle mass index, physical fitness, and quality of life among older people with sarcopenia. Geriatrics. 2018;18:17.
Hortobágyi T, Lesinski M, Fernandez-del-Olmo M, Granacher U. Small and inconsistent effects of whole body vibration on athletic performance: a systematic review and meta‑analysis. Eur J Appl Physiol. 2015;115(8):1605-1625.
Talmadge RJ, Roy RR, Caiozzo VJ, Edgerton VR. Mechanical properties of rat soleus after long-term spinal cord transection. J Appl Physiol. 2002;93(4):1487-1497.
Ferreira R, Neuparth MJ, Ascensão A, Magalhães J, Duarte J, Amado F. Atrofia muscular esquelética. Modelos experimentais, manifestações teciduais e fisiopatologia. Rev Port Ciências do Desporto. 2004;4(3):94111.
Marcotte GR, West DWD, Baar K. The molecular basis for load-induced skeletal muscle hypertrophy. Calcif Tissue Int. 2015;96(3):196-210.
Taillandier D, Aurousseau E, Combaret L, Guezennec C-Y, Attaix D. Regulation of proteolysis during reloading of the unweighted soleus muscle. Int J Biochem Cell Biol. 2003;35(5):665-675.
Durigan JLQ, Cancelliero KM, Dias CNK, Silva CA da, Guirro RR de J, Polacow MLO. Estudo morfométrico do músculo sóleo de ratos submetidos à imobilização aguda associado à estimulação elétrica neuromuscular. Fisioter em Mov. 2006;19(2):117-126.
Polizello JC, Carvalho LC, Freitas FC, Padula N, Martinez EZ, Mattiello-Sverzut AC. Efeitos morfológicos do retorno da sobrecarga após imobilização em alongamento de músculo esquelético de ratas. Rev Bras Fisioter. 2011;15(1):73-79.
Pucciarelli MLR, Mattiello SM, Martinez EZ, Mattiello-Sverzut AC. Exercício excêntrico e alongamento para músculos flexores plantares aplicados durante 21 dias após imobilização não modificam o tecido não contrátil. Fisioter e Pesqui. 2016;23(2):118-123.
Shah SB, Davis J, Weisleder N, et al. Structural and functional roles of desmin in mouse skeletal muscle during passive deformation. Biophys J. 2004;86(5):2993-3008.
O’Neill A, Williams MW, Resneck WG, Milner DJ, Capetanaki Y, Bloch RJ. Sarcolemmal organization in skeletal muscle lacking desmin: evidence for cytokeratins associated with the membrane skeleton at costameres. Mol Biol Cell. 2002;13(7):2347-2359.
Zepetnek JOT De, Giangregorio LM, Craven BC. Whole-body vibration as potential intervention for people with low bone mineral density and osteoporosis: A review. J Rehabil Res Dev. 2009;46(4):529-542.
Wiggs MP. Can endurance exercise preconditioning prevention disuse muscle atrophy? Front Physiol. 2015;6:63.
Qaisar R, Bhaskaran S, Van Remmen H. Muscle fiber type diversification during exercise and regeneration. Free Radic Biol Med. 2016;98:56-67.
Otto A, Collins-Hooper H, Patel K. The origin, molecular regulation and therapeutic potential of myogenic stem cell populations. J Anat. 2009;215(5):477-497.
Cardinale M, Bosco C. The use of vibration as an exercise intervention. Exerc Sport Sci Rev. 2003;31(1):3-7.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2019 European Journal of Clinical and Experimental Medicine
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our open access policy is in accordance with the Budapest Open Access Initiative (BOAI) definition: this means that articles have free availability on the public Internet, permitting any users to read, download, copy, distribute, print, search, or link to the full texts of these articles, crawl them for indexing, pass them as data to software, or use them for any other lawful purpose, without financial, legal, or technical barriers other than those inseparable from having access to the Internet itself.
All articles are published with free open access under the CC-BY Creative Commons attribution license (the current version is CC-BY, version 4.0). If you submit your paper for publication by the Eur J Clin Exp Med, you agree to have the CC-BY license applied to your work. Under this Open Access license, you, as the author, agree that anyone may download and read the paper for free. In addition, the article may be reused and quoted provided that the original published version is cited. This facilitates freedom in re-use and also ensures that Eur J Clin Exp Med content can be mined without barriers for the research needs.
