Expression of collagen type III and type IV in breast cancer

Authors

  • Anna Goździalska Z Zakładu Analityki Biochemicznej Wydziału Farmaceutycznego z Oddziałem Analityki Medycznej Collegium Medicum Uniwersytetu Jagiellońskiego
  • Anna Gawędzka Z Zakładu Analityki Biochemicznej Wydziału Farmaceutycznego z Oddziałem Analityki Medycznej Collegium Medicum Uniwersytetu Jagiellońskiego
  • Jagoda Drąg Z Zakładu Analityki Biochemicznej Wydziału Farmaceutycznego z Oddziałem Analityki Medycznej Collegium Medicum Uniwersytetu Jagiellońskiego
  • Małgorzata Knapik-Czajka Z Zakładu Analityki Biochemicznej Wydziału Farmaceutycznego z Oddziałem Analityki Medycznej Collegium Medicum Uniwersytetu Jagiellońskiego
  • Anna Siekanowicz Z Zakładu Analityki Biochemicznej Wydziału Farmaceutycznego z Oddziałem Analityki Medycznej Collegium Medicum Uniwersytetu Jagiellońskiego
  • Adrian Kużdżał Z Instytutu Fizjoterapii Wydziału Medycznego Uniwersytetu Rzeszowskiego; Z Krakowskiej Wyższej Szkoły Promocji Zdrowia https://orcid.org/0000-0002-5685-3139
  • Jerzy Jaśkiewicz Z Zakładu Analityki Biochemicznej Wydziału Farmaceutycznego z Oddziałem Analityki Medycznej Collegium Medicum Uniwersytetu Jagiellońskiego

Keywords:

breast cancer, collagen type III, collagen type IV

Abstract

Introduction: Breast cancer is the most common cause of cancer death in women in Poland and it is the second most frequent cancer in women worldwide. The structure of both healthy as well as tumor breast tissue is maintained by extracellular matrix components including collagens. The rate of collagens degradation depends on matrix metalloproteinases (MMPs) activities. In non tumor tissue the turnover rate of extracellular matrix is the crucial factor in preserving the proper function of the breast tissue, while in cancer it reflects the dynamics of tumor cell infiltration. The main objective of our study was to determine the expression of collagen type III and type IV proteins in breast tissue obtained from women with diagnosed breast cancer.

Materials and Methods: From each patient two specimens of breast tissue were taken during mastectomy, one from the tumor center and the second from the bordering healthy tissue (control group). Expression of both types of collagen proteins was evaluated by Western blot technique with chemiluminescence detection. The results obtained for collagen III and IV of the tumor and nontumor tissue, were expressed as a mean value ± SD of collagen expression. D'Agostino test for normality of distribution was assessed. The parametric test (t-Student's test) and Wilcoxon pair sequence were used to verify the results.

Results: The results of this study demonstrated the expression of type III and type IV collagen proteins in both tumor and non tumor breast tissue. The expression of different types of collagen according to age was demonstrated. Patients in the group up to 55 years of age found a higher expression of type IV collagen in the nontumor tissues, while in the group above 55 years of age the expression of collagen IV was higher in the tumor. Statistically significant differences in the expression of collagen type IV between tumor and non tumor tissue, in only the group over 55 years of age were found.

Conclusions: The different expression of collagen proteins in tumor and nontumor tissues may be one of the markers used in breast cancer diagnostics.

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References

Kozak A., Sielużycka J., Pronobis J., Ząbkowska K., Górnasiowa M., Pawlak W.Z.: Genetic testing for hereditary breast and ovarian cancer susceptibility, Współcz Onkol 2002; 6:201–204.

Bednarek A., Płuciennik E., Kordek R., Kusińska R.: New molecular prognostic factors in patients with breast cancer, Współcz Onkol 2004; 8:296–302.

Michels K.B., Mohllajee A.P., Roset- Bahmanyar E., Beehler G.P., Moysich K.B.: Diet and breast cancer. A review of the prospective observational studies, Cancer 2007; 109:2712– 2749.

Li C.I., Daling J.R., Malone K.E., Bernstein L., Marchbanks P.A., Liff J.M., et al.: Relationship between established breast cancer risk factors and risk of seven different histologic types of invasive breast cancer, Cancer Epidemiol Biomarkers Prev 2006; 15: 946–954.

Makowski M., Połać I., Pertyński T.: Estrogeny a rak sutka, Przegląd Menopauzalny 2007; 11:150–154.

Provenzano P.P., Inman D.R., Eliceiri K.W., Knittel J.G., Yan L., Rueden C.T., White J.G., Keely P.J.: Collagen density promotes mammary tumor initiation and progression, BMC Med 2008; 6:11.

Baluk P., Morikawa S., Haskell A., Mancuso M., McDonald D.M.: Abnormalities of basement membrane on blood vessels and endothelial sprouts in tumors. Am J Pathol 2003; 163: 1801–1815.

Mazouni C., Arun B., André F., Ayers M., Krishnamurthy S., Wang B. et al.: Collagen IV levels are elevated in the serum of patients with primary breast cancer compared to healthy volunteers, Br J Cancer 2008; 99: 68–71.

Xu J., Rodriguez D., Petitclerc E., Kim J.J., Hangai M., Moon Y.S., Davis G.E., Brooks P.C.: Proteolytic exposure of a cryptic site within collagen type IV is required for angiogenesis and tumor growth in vivo, J Cell Biol 2001; 154:1069–1080.

Liu S.C., Yang S.F., Yeh K.T., Yeh C.M., Chiou H.L., Lee C.Y., Chou M.C., Hsieh Y.S.: Relationships between the level of matrix metalloproteinase-2 and tumor size of breast cancer, Clin Chim Acta 2006; 371:92–96.

Kass L., Erler J.T., Dembo M., Weaver V.M.: Mammary epithelial cell, influence of extracellular matrix composition and organization during development and tumor genesis, J Biochem Cell Biol 2007; 39:1987–94.

Jarocka-Cyrta E., Bańkowski E.: Zawartość i polimorfizm molekularny kolagenu w sutku objętym procesem łagodnej dysplazji, Polski Tygodnik Lekarski 1991; 46:981–983.

Egeblad M., Werb Z.: New functions for matrix metalloproteinases in cancer progression, Nat Rev 2002; 2:161–174.

Murphy G., Nagase H.: Progress in matrix metalloproteinase research. Mol Aspects Med 2008; 29:290–308.

McCaw A., Ewald A.J., Werb Z.: Matrix metalloproteinases and the regulation of tissue remodeling, Nature Reviews Molecular Cell Biology 2007; 8: 221–233.

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Published

2010-06-30

How to Cite

Goździalska, A., Gawędzka, A., Drąg, J., Knapik-Czajka, M., Siekanowicz, A., Kużdżał, A., & Jaśkiewicz, J. (2010). Expression of collagen type III and type IV in breast cancer. European Journal of Clinical and Experimental Medicine, 8(2), 128–133. Retrieved from https://journals.ur.edu.pl/ejcem/article/view/13056

Issue

Vol. 8 No. 2 (2010): Medical Journal of the Rzeszow University

Section

ORIGINAL PAPERS

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Copyright (c) 2010 Medical Journal of the Rzeszow University

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